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Reason: All Data are in SILS tape archive 2016_Breit_mother.spec.mat.transcr.zfeggs_Rauwerda_Breit
Mother-Specific Signature in the Maternal Transcriptome Composition of Mature,Unfertilized Zebrafish Eggs
dataset
posted on 2020-02-17, 11:05 authored by J. Rauwerda, Timo M. BreitMaternal mRNA
present in mature oocytes plays an important role in the proper
development of the early embryo. As the composition of the maternal
transcriptome in general has been studied with pooled mature eggs,
potential differences between individual eggs are unknown. Here we
present a transcriptome study on individual zebrafish eggs from clutches
of five mothers in which we focus on the differences in maternal mRNA
abundance per gene between and within clutches. To minimize technical
interference, we used mature, unfertilized eggs from siblings. About
half of the number of analyzed genes was found to be expressed as
maternal RNA. The expressed and nonexpressed genes showed that maternal
mRNA accumulation is a non-random process, as it is related to
specific biological pathways and processes relevant in early
embryogenesis. Moreover, it turned out that overall the composition of
the maternal transcriptome is tightly regulated as about half of the
expressed
genes display a less than twofold expression range between the observed
minimum and maximum expression values of a gene in the experiment. Even
more, the maximum gene-expression difference within clutches is for 88%
of the expressed genes lower than twofold. This means that expression
differences observed in maternally expressed genes are primarily caused
by differences between mothers, with only limited variability between
eggs from the same mother. This was underlined by the fact that 99% of
the expressed genes were found to be differentially expressed between
any of the mothers in an ANOVA test. Furthermore, linking chromosome
location, transcription factor binding sites, and miRNA target sites of
the genes in clusters of distinct and unique mother-specific
gene-expression, suggest biological relevance of the mother-specific
signatures in the maternal transcriptome composition.
Altogether, the maternal transcriptome composition of mature zebrafish
oocytes seems to be tightly regulated with a distinct mother-specific
signature.
GSE72839at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?accGSE72839,
and http://rnabiology.nl/Dr-Browser.html
2 papers:
Mother-Specific Signature in the Maternal Transcriptome Composition of
Mature, Unfertilized Zebrafish Eggs. Rauwerda H, Wackers P, Pagano JF,
de Jong M, Ensink W, Dekker R, Nehrdich U, Spaink HP, Jonker M, Breit
TM. PLoS One. 2016 Jan 22;11(1):e0147151. doi:
10.1371/journal.pone.0147151. eCollection 2016. PMID: 26799215
Transcriptome data on maternal RNA of 24 individual zebrafish eggs from
five sibling mothers. Pagano JF, Rauwerda H, de Leeuw WC, Wackers P, de
Jong M, Ensink W, Dekker R, Nehrdich U, Spaink HP, Jonker M, Breit
TM.Data Brief. 2016 Apr 26;8:69-72. doi: 10.1016/j.dib.2016.04.045.
eCollection 2016 Sep. PMID: 27284564
All Data are in SILS tape archive 2016_Breit_mother.spec.mat.transcr.zfeggs_Rauwerda_Breit
History
Retention period
2031-03-01Research priority area
- Systems Biology